You are here

Головна » 2025 » AGROBIOLOGY №1 2025

Induction of rhizogenesis in Prunus dulcis (Mill.) D.A. Webb by nutritional and hormonal determinants

Almond (Prunus dulcis) is one of the promising nut-bearing crops capable of mitigating climate risks to achieve sustainable development goals and prevent threats to agroecological and food security. For the rapid propagation of high-quality planting material, microclonal propagation is the most suitable method, carried out under controlled conditions in four stages. The success of rhizogenesis (one of the stages) determines further survival of regenerants during the adaptation phase. The study was carried out under standard conditions in the interdepartmental plant biotechnology laboratory of BNAU. Explants of four domestic sweet almond cultivars («E5 Borozan», «M 41 Alex», «Georgia», «Louise») were used in the research. We studied the influence of nutritional and hormonal determinants on sweet almond rhizogenesis. The results demonstrate varietal specificity of almond reactions to auxin type, phytohormones ratio, and other technological determinants. The study of the effect of synthetic auxin analogues (indolebutyric, indoleacetic, naphthylacetic acids) on the induction of rhizogenesis has established that the optimal conditions for root formation are the use of indolebutyric acid at a concentration of 0.75 mg/l in combination with 0.125 mg/l BAP. The concentration of sucrose (1–2 %) promotes the formation of a developed root system and improves the biometric indicators of regenerants. Higher sucrose concentrations led to tissue hyperhydration and callus formation, complicating plant adaptation. The effect of mesoinositol and activated charcoal was also studied, revealing that moderate concentrations improved root formation. The optimal conditions were determined to be the addition of 2% sucrose and 1.0–1.5 g/L of activated charcoal. Therefore, the research results showed that the effectiveness of sweet almond rhizogenesis in vitro depends on many factors, among which the type of explant, the hormonal ratio in the medium, and its trophic composition. Optimization of these parameters at the stage of rhizogenesis is key to increasing productivity in microclonal propagation of this crop.

Key words: rhizogenesis, in vitro, almond, phytohormones, nutrient medium, rooting, sucrose.

Shita О. https://orcid.org/0000-0002-6470-2744


L. Filipova https://orcid.org/0000-0002-7447-5418


V. Matskevych https://orcid.org/0000-0002-9314-8033


Reference: 
1. Matskevich, V., Kimeichuk, I., Matskevich, О., Shita, О. (2022). Svitovyi dosvid, perspektyvy v Ukraini rozmnozhennia funduka ta myhdaliu [Global experience and prospects in Ukraine for the propagation of hazelnut and almond]. Ahrobiolohiia: zbirnyk naukovykh prats [Agrobiology]. Bila Tserkva, BNAU, no. 1, pp. 179–191.
2. Matskevych, V.V., Filipova, L.M., Oleshko, O.H. (2022). Fiziolohiia i biotekhnolohiia roslyn [Physiology and biotechnology of plants]. Bila Tserkva, BNAU, 602 р.
3. Filipova, L.M., Matskevych, V.V., Shyta O.P. (2021). Trofichni determinanty ontohenezu rehenerantiv myhdaliu in vitro [Trophic determinants of ontogenesis of almond regenerants in vitro]. Henetyka i selektsiia v suchasnomu ahrokompleksi: materialy VI Vseukrainskoi naukovo-praktychnoi konferentsi [Genetics and breeding in the modern agrocomplex: materials VI All-Ukrainian Scientific and Practical Conference]. Uman, рр. 202–204.
4. Shyta, O.P. (2022). Determinanty rozmnozhennia Rrunus dulcis (mill.) D.a.webb. biotekhnolohichnymy metodamy [Determinants of Prunus dulcis (Mill.) D.A. Webb propagation by biotechnological methods].
Ahrobiolohiia: zbirnyk naukovykh prats [Agrobiology]. Bila Tserkva, BNAU, no. 2, рр.137–152.
5. Shyta, O.P., Filipova, L.M., Matskevych, V.V. (2024). Osoblyvosti multyplikatsii in vitro kistochkovykh kultur: zbirnyk naukovykh prats. [Peculiarities of in vitro multiplication of stone fruit crops]. Ahrobiolohiia [Agrobiology]. Bila Tserkva, BNAU, no. 1, рр. 222–236.
6. Tereso, S., Miguel, C., Mascarenhas, M., Roque, A., Trindade, H., Maroco, J., Oliveira, M. (2008). Improved in vitro rooting of Prunus dulcis Mill. cultivars. Biologia Plantarum. no. 52, pp. 437– 444. DOI: 10.1007/s10535-008-0088-2.
7. Ainsley, P.J., Collins, G.G., Sedgley, M. (2001). In vitro rooting of almond (Prunus dulcis Mill.). In Vitro Cell. Dev. Biol.-Plant. no. 37, pp. 778–785. DOI: 10.1007/s11627-001-0129-4.
8. Kodad, S., Melhaoui, R., Hano, C., Addi, M., Sahib, N., Elamrani, A., Abid, M., Mihamou, A. (2021). Effect of Culture Media and Plant Growth Regulators on Shoot Proliferation and Rooting of Internode Explants from Moroccan Native Almond (Prunus dulcis Mill.) Genotypes. International Journal of Agronomy. 10 p. DOI: 10.1155/2021/9931574].
9. Damiano, C., Archilletti, T., Caboni, E., Lauri, P., Falasca, G., Mariotti, D., Ferraiolo, G. (1994). Agrobacterium mediated transformation of almond: in vitro rooting through localized infection of A. rhizogenes wt. Genetic Improvement of Horticultural Crops by Biotechnology. no. 392, pp. 161–170. DOI: 10.17660/ActaHortic.1995.392.19
10. Caboni, E., Tonelli, M., Lauri, P. (1997). Biochemical aspects of almond microcuttings related to in vitro rooting ability. Biologia Plantarum. no. 39, pp. 91–97. DOI: 10.1023/A:1000365224324
11. Koaym, W., Twaklna, M., AlMattar, E. (2022). Application of plant tissue culture technique to micropropagation of the most important almond cultivars in Syria. Journal of Genetic and Environmental Resources Conservation. no. 10(3), pp. 225–234.
12. Cheong, E.J., An, C. (2015). Effect of Carbohydrates on in vitro Shoot Growth of Various Prunus Species. Korean Journal of Plant Resources. no. 28(3), pp. 357–362. DOI: 10.7732/KJPR.2015.28.3.357
13. Sevde, K., Fatih, A. (2015). CANLI*In vitro Propagation of ‘Garnem’ (P. persica x P. dulcis) Rootstock. J Plant Molecular Biology & Biotechnology. no. 5(1), pp. 25–30. Available at: https://www.researchgate.net/profile/Fatih_Canli/publication/286264820_ In_vitro_Propagation_of_'Garnem'_P_persica_x_P_ dulcis_Rootstock/links/5667699e08aea62726ee8fdf/ In-vitro-Propagation-of-Garnem-P-persica-x-P-dulcis-Rootstock.pdf
14. Suleimanova, S. (2022). Improvement of in vitro rooting of microcuttings some rootstocks of fruit crops. Research in: Agricultural & Veterinary Sciences. Vol. 6, no. 1, pp. 22–27. Available at: https://agris.fao.org/search/en/providers/123799/records/647478d4425ec3c...
15. Kushnir, H.P., Sarnatska, V.V. (2005). Mikroklonalne rozmnozhennia roslyn [Microclonal propagation of plants]. Kyiv, Scientific thought, 271 р.
16. Matskevych, V.V., Kravchenko, N.V., Podhaietskyi, A.A. (2023). Mikroklonalne rozmnozhennia roslyn [Microclonal propagation of plants]. Sumy, 215 p.
17. Okhorona prav na sorty roslyn: biuleten [Protection of plant variety rights]. Ukrainskyi instytut ekspertyzy sortiv [Ukrainian Institute of Variety Examination]. Vinnytsia, LLC «TVORY», 2020, Vol. 5, 395 p.
18. Kors, F.T.M. Plant cell and tissue culture. Phytopathology. Biochemicals. Catalogye 2010– 2012. Duchefa Biochemie B.V. 194 p. Available at: http://brochure.duchefa-biochemie.com/ Duchefa_ catalogus_2010_2012/ .
19. Murashige, T., Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology. no. 15, pp. 473–497. DOI: 10.1111/j.1399-3054.1962. tb08052.x/.
20. Nas, M., Read, R. (2004). A hypothesis for the development of a defined tissue culture medium of higher plants and micropropagation of hazelnuts. Scientia Horticulturae. no. 101, pp. 189–200. DOI: 10.1016/j.scienta.2003.10.004.
21. Horvat, A.A., Molnar, O.O., Minkovych, V.V. (2019). Metody obrobky eksperymentalnykh danykh z vykorystanniam MS Excel: navch. posib. [Methods of processing experimental data using MS Excel]. Uzhhorod, Publishing House of Uzhhorod National University "Hoverla", 160 р.
22. Matskevych, V.V. (2020). Mikroklonalne rozmnozhennia vydiv roslyn in vitro ta yikh postaseptychna adaptatsiia: dys. d-ra s.-h. nauk: 06.01.05 [Microclonal propagation of plant species in vitro and their postaseptic adaptation: diss. Dr. of agricultural Sciences: 06.01.05]. Sumy, 478 р.
23. Shyta, O.P., Filipova, L.M., Matskevych, V.V. (2024). Osoblyvosti zahalnoi stratehii zhyvtsiuvannia myhdaliu in vitro: IV Mizhnarodna naukovo-praktychna internet-konferentsiia «Suchasni vyklyky i aktualni problemy lisivnychoi osvity, nauky ta vyrobnytstva» [Features of the general strategy of in vitro grafting of tonsils: IV International Scientific and Practical Internet Conference "Modern Challenges and Topical Problems of Forestry Education, Science and Production"]. Bila Tserkva, BNAU, 215 p.
24. Makrushyn, M.M., Makrushyna, Ye.M., Peterson, N.V., Melnykov, M.M. (2006). Fiziolohiia roslyn: pidruchnyk [Plant physiology]. Vinnytsia, New book, 416 p.
25. Vedenychova, N.P., Kosakivska, I.V. (2017). Tsytokininy yak rehuliatory ontohenezu roslyn za riznykh umov zrostannia [Cytokinins as regulators of plant ontogenesis under different growth conditions]. Kyiv, Our format, 200 р.
26. Terek, O.I., Patsula, O.I. (2011). Rist i rozvytok roslyn: navch. posib. [Growth and development of plants]. Lviv, LNU named after Ivan Franko, 328 p.
27. Matskevych, V.V., Podhaietskyi, A.A., Filipova, L.M. (2019). Mikroklonalne rozmnozhennia okremykh vydiv roslyn (protokoly tekhnolohii): nauk.-prakt. posib. [Microclonal propagation of selected plant species (technology protocols)]. Bila Tserkva, BNAU, 85 p.
28. Matskevych, V., Yukhnovskyi, V., Kimeichuk, I., Matskevych, O, Shyta, O. (2023). Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture. Folia Forestalia Polonica. Vol. 65, no. 1, pp. 1–14. DOI: 10.2478/ffp-2023-0001
29. Kobyletska, M.S., Terek, O.I. (2017). Biokhimiia roslyn: navch. posib. [Biochemistry of plants]. Lviv, 269 р.
30. Kobyletska, M.S., Patsula, O.I., Romaniuk, N.D., Terek, O.I., Baranov, V.I., Mamchur, O.V. (2023). Fiziolohiia ta biokhimiia roslyn [Plant physiology and biochemistry]. Lviv, Vol. 1, LNU im. Ivana Franka, 378 p.
31. Skliar, V.H. (2015). Ekolohichna fiziolohiia roslyn: pidruchnyk [Ecological plant physiology]. Sumy, University book, 271 p.
Download this article: 
AttachmentSize
PDF icon shyta_1_2025.pdf1020.66 KB
YEAR: 
2025
AGROBIOLOGY №1 2025