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Stability of Rhizobium leguminosarum–Pisum sativum symbiosisb under the action of PPC under conditions of artificial drought

Considering climate change and frequent droughts, it is urgent to find ways to increase the survival of Rhizobium leguminosarum in the periseed zone during pre-sowing seed inoculation, which will ensure the formation of a more stable legumerhizobial symbiosis. The effect of a polysaccharide-protein complex (PPC) on the efficiency of seed inoculation with active strains of R. leguminosarum under conditions of insufficient moisture was studied. The survival of nodule bacteria after bacterization was compared and its effect on plant development and nitrogenfixing activity was assessed under optimal (60 % total moisture capacity, VC) and low (30 % VC) moisture conditions. The aim of the study was to determine the survival of nodule bacteria in the peri-seed zone and assess the effect of bacterization of pea seeds (Pisum sativum L.) with PPC on the formation and mass of nodules, nitrogen fixation, and initial plant growth under artificial drought. Methods used: microbiological, physiological, chromatographic and statistical. The data obtained showed that inoculation of pea varieties Tsarevich with active strains of R. leguminosarum has a positive effect on plant growth. A correlation between bacterial survival in the peri-seed zone on the moisture level and the inoculant strain was revealed. 
When bacterization was combined with PBC, bacterial survival was higher, which was accompanied by a greater mass of nodules and higher nitrogen fixation. It was determined that all indicators of the functioning of the R. leguminosarum – Pisum sativum symbiosis during drought decreased compared to optimal conditions. The most effective was the inoculation of pea seeds of the Tsarevich variety with the R. leguminosarum G222 strain in combination with PBC, which provided the greatest increase in the number and mass of nodules, as well as high nitrogen fixation rates both under optimal and insufficient moisture.

Key words: Rhizobium leguminosarum, biological nitrogen fixation, legume-rhizobial symbiosis, artificial drought, polysaccharide-protein complex (PPC).

Voronaia O. https://orcid.org/0000-0001-6029-4758


Kozar S. https://orcid.org/0000-0003-1341-5603


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AGROBIOLOGY №2 2025