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Molecular-genetic characteristics of raspberry varieties based on ISSR-PCR markers

For the first time 12 raspberry varieties cultivated in Ukraine were analyzed for their genetic structure using ISSR-PCR markers, and the effectiveness of using this type of marker for studying genetic polymorphism and the relatedness of raspberry varieties was evaluated. DNA extraction was performed from fresh plant material using young raspberry leaves. DNA was extracted using a CTAV buffer. In order to select informative ISSR markers for raspberry genome analysis, 31 primers were tested – 21 with a dinucleotide cow sequence and 10 with a trinucleotide sequence. Based on the results of primer screening, four dinucleotide ISSR primers, (AG)8 YG, (AG)9 C, (AC)8 C, and (AG)9 C, and three trinucleotide primers, (ACC)6 G, (GTG)6 A, and (GAG)6 G, were selected for further molecular-genetic analysis of varietal polymorphism. A high level of polymorphism of ISSR markers was identified in raspberries. The average number of alleles per locus was 1.795, the average effective number of alleles per locus was 1.44, the average Shannon diversity index was 0.398, and the average 37 agrobiologiya.btsau.edu.ua Агробіологія, 2025, № 2 expected heterozygosity was 0.262. The range of genetic distances between the studied varieties ranged from 0.109 to 0.606, and genetic relatedness indices ranged from 0.896 to 0.545. It was concluded that the ISSR-PCR multilocus DNA profiling method is highly informative for the genetic identification of raspberry varieties. The ISSR markers studied may be useful for characterizing the genetic structure, distinguishing between raspberry varieties, and selecting the most promising options for crossing.

Key words: raspberry, Rubus idaeus L., polymorphism, ISSR-PCR markers, genetic differentiation.

Dyman N.


Karpuk L. https://orcid.org/0000-0002-5860-5286


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AGROBIOLOGY №2 2025