You are here

Creation and storage of oats collection when biotechnological technique is used

The research was carried out to identify some peculiar conditions for the formation of an active collection of oats in vitro and the creation of a balanced composition of culture medium with the content of various mineral and hormonal components, which would ensure a long-term and economically expedient storage of cultural plants of oats at an optimal temperature regime.

Cultural oats plants of a different breeding direction in a non-rooted condition were used for deposition.

The conducted research aimed at the improvement of the composition of culture medium for deposition has proved that it is advisable to use medium by the recommendation of Hamborh and Eveleh (GB) with the addition of BAP (benzylaminopurine). Based on the data received it was possible to state that BAP concentration – 0,3 mg/l, which was added to culture medium, was the most optimal one, and the increase of BAP concentration to 0,5 mg/l resulted in the most active oats sprout formation, which was not acceptable in the process of the collection storage in vitro. The research confirmed that BAP concentration 0.1 mg/l and 0.2 mg/l had no effect on oats sprout formation.

The effect of cytokinins and carbohydrates was studied, their optimal concentrations in the composition of culture medium were chosen. It has been proved experimentally that it is not advisable to use kinetin for oats regardless of the concentrations because it causes the formation of additional sprouts which is not desirable in this case. The addition of sucrose and glucose into culture medium at concentrations from 30 to 80 g/l showed that regardless of glucose concentrations the indicators of a height and number of sprouts were higher as compared with sucrose, which was not good in this storage process of oats collection. It has been established that at sucrose concentration 70–80 g/l cultural oats plants are 16–25 cm high and have 12–15 sprouts, but after a long-term storage, after 6 months, they become more suppressed and a higher percentage of necrosis-affected pants is observed. The most efficient sucrose concentration ranges from 40 to 50 g/l, it allows to get the number of sprouts 5–7 pieces and a height of cultural oats plants 7–9 cm.

One of the most important factors of plant deposition is the effect of low positive temperatures. Temperature regimes from +6 to +16 °С, at which a condition and a period of plant material storage were studied, showed that temperatures +6 °С and +8 °С during a long-term storage facilitated the plants of oats parent material in vitro to undergo the stage of vernalization which was an undesirable phenomenon during deposition.

The following was received at temperature regime +10 °С: a slight sprout increase 0.8±0.2 cm, a plant height 7 cm, and the number of sprouts 3–4 pieces; it turned out to be the most optimal among the studied temperatures.

A collection storage period showed that a high percentage of cultural oats was recorded in the 8th and 10th months of storage; this indicator was 89 % and 82 %, respectively. At temperature +10 °С and +8 °С and +6 °С during four months of deposition, plant material remained in an unchanged state and 100 % were stored; however, a considerable decrease in the amount of plant material occurred after a longer storage.

The preservation of the oats collection at temperature regimes +14 °С and +12 °С and 65 and 55 days of active growth was at level 42 and 45 % of cultural plants after 12 months of storage.

It has to be stated that the percentage of plants during a storage period decreased seriously after 10 months of deposition almost in all studied variants. It has been established that the most optimal positive temperature to store parent oats material is + 10 °С, which ensures up to 69.5 % of plant preservation.

Key words: culture medium, temperature regime, concentrations, cytokinins, carbohydrates, storage duration.



1. Stankevych, H.M., Koropenko, S.V. (2008). Holozernyi oves – perspektyvna kultura dlia kombikormovoi haluzi [Naked oat: a promising crop for complex feed production]. Khranenie i pererabotka zerna [Grain Storage and Processing], no. 7, pp. 42–44.

2. Cherchel, V.Yu., Fedorenko, E.M., Aldoshyn, A.V., Solodushko, V.P., Liashenko, N.O. (2014). Oves – stan ta efektyvnist vyrobnytstva, novi sorty i mozhlyvosti [Oat: state of art and production efficiency, new varieties and opportunities]. Selektsia i nasinnitstvo [Plant Breeding and Seed Production], vol. 106, pp. 183–190.

3. Boiko, V.I., Lebid, Ye.M., Rybka, V.S. et al. (2008). Ekonomika vyrobnytstva zerna (z osnovamy orhanizatsii i tekhnolohii vyrobnytstva) [The economy of grain production (with the basics of organization and production technology)]. Kyiv, NNTs IAE, 400 p.

4. Belokurova, V.B. (2010). Metody biotekhnolohii v systemi zakhodiv zi zberezhennia bioriznomanittia roslyn [Methods of biotechnology in the system of efforts for plant biodiversity preservation]. Tsitol Genet. [Cytol. Genet.], vol. 44, no. 3, pp. 58–72.

5. Shpak, L.M., Rakhmetov, D.B., Levenko, B.A. (2012). Dlitel'noe khranenie Stevia rebaudiana Bert. v kul'ture in vitro [Long-term storage of Stevia rebaudiana Bert. in vitro]. Problemy eksperymentalnoi botaniky ta biotekhnolohii [Issuess of Experimental Botany and Biotechnology]. Kyiv, Fitosotsiotsentr, pр. 58–85.

6. Molkanova, O.I., Kotorkov, O.I., Vetchenkina, E.M., Mamaeva, N.A., Vasil'eva, O.G. (2010). Geneticheskie banki rasteniy: problemy formirovaniya, sokhraneniya i ispol'zovaniya [The gene banks: problems of formation, preservation and use]. Vestnik Udmurtskogo universiteta. Seriya: Biologiya. Nauki o zemle [Bulletin of Udmurt University. Series Biology. Earth Sciences], vol. 3, pp. 33–39.

7. Reed, B.M., Sarasan, V., Kane, M., Bunn, E., Pence, V.C. (2011). Biodiversity conservation and conservation biotechnology tools. In Vitro Cell. Dev. Biol. Plant., vol. 47, no. 1, pp. 1–4. Retrieved from:

8. Engelmann, F. (2011). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell. Dev. Biol. Plant., vol. 47, no. 1, pp. 5–16. Retrieved from:

9. Rakosy-Tican, E., Bors, B., Szatmari, A.M. (2012). In vitro culture and medium-term conservation of the rare wild species Gladiolus imbricatus. Afr. J. Biotechnol., vol. 11, pp. 14703–14712. Retrieved from:

10. Voitovska, V.I., Nediak, T.M., Necheporenko, L.P. (2013). Stvorennia kolektsii vivsa u kulturi in vitro [Building-up oat collection in vitro]. Pidvyshchennia efektyvnosti resursozberihaiuchykh tekhnolohii na zernopererobnykh pidpryiemstvakh: tezy dopovidei Vseukr. nauk. konf. [Increasing the efficiency of resource-saving technologies at grain processing enterprises]. Uman, VPTs “Vizavi”, рр. 54–55.

11. Storozhik, L.I., Voytovskaya, V.I., Nedyak, T.N. (2015). Vegetativnoe razmnozhenie sorgo sakharnogo [Vegetative propagation og sugar sorghum]. Zemledelie i zashchita rasteniy [Agriculture and Plant Protection], no. 3, pp. 18–22.

12. Belokurova, V.B., Kuchuk, N.V. (2014). In vitro bank and seed collection of wild-growing plants as a tool for plant conservation and utilization in biotechnological studies. Behl, R.K., Arseniuk, E. eds. Biotechnology and Plant Breeding Perspectives. Jodhpur, India, Agrobios (Int.), Babloo Offset, pp. 219–232.

13. Koval', S.F., Koval', V.S., Tymchuk, S.M., Boguslavskiy, R.L. (2003). Geneticheskie kollektsii: problemy formirovaniya, sokhraneniya i ispol'zovaniya [Genetic collections: problems of building-up, maintenance and usage].Tsitol Genet. [Cytol. Genet.], vol. 37, no. 4, pp. 46–53.

14. Gaspar, T., Kevers, C., Debergh, P. Vitrification: morphological, physiological and ecological aspects. Cell and Tissue Culture in Forestry. Dordrecht, Holland : Martinus Nijhoff Publ. 1987, Vol. I, pp. 152–166.

15. Rao, N.K. Plant genetic resources: Advancing conservation and use through biotechnology. Afr. J. Biotechnol. 2004, Vol. 3, no. 2, pp. 136–145. Retrieved from:

16. Bairu M.W., Stirk W.A., van Staden J. Factors contributing to in vitro shoot-tip necrosis and their physiological interactions. Plant Cell Tissue Organ Cult. 2009, Vol. 98, no. 3, pp. 239–248. Retrieved from:

17. Altpeter, F., Posselt, U.K. Improved plant regeneration from cell suspensions of commercial cultivars, breeding and inbred lines of perennial rye grass (Lolium perenne L.). J. Plant Physiol. 2000, Vol. 156, no. 5–6, pp. 790–796. Retrieved from:

18. Burgess, T.L., Blazich, F.A., Nash D.L. Influence of stratification, temperature, and light on seed germination of southern sea oats. SNA Research Conference. 2001, Vol. 46, pp. 394–397.

19. Cui, S.X., Wang, W., Zhang, C.L. Plant regeneration from callus cultures in two ecotypes of reed (Phragmites  communis Trinius). In Vitro Cell. Dev. Biol. Plant. 2002, Vol. 38, no. 4, pp. 325–329. Retrieved from:

20. Hu, X.R., Yang, A.F., Zhang, K.W., Wang, J., Zhang, J.R. Optimization of in vitro multiple shoots clump induction and plantlet regeneration of Kentucky bluegrass (Poa pratensis). Plant Cell Tissue Organ Cult. 2006, Vol. 84, no. 1, pp. 90–99. Retrieved from:

21. Valero-Aracama, C., Kane, M.E., Wilson, S.B., Philman, N.L. Genotypic differences of in vitro propagated sea oats. SNA Research Conference. 2002, Vol. 47, pp. 357–360.

22. Valero-Aracama, C., Kane, M.E., Wilson, S.B., Vu, J.C., Anderson, J., Philman, N.L. Photosynthetic and carbohydrate status of easy- and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during in vitro culture and
ex vitro acclimatization. In Vitro Cell Dev. Biol-Plant. 2006, Vol. 42, no. 6, pp. 572–583. Retrieved from:

23. Bhatt, I.D., Dhar, U. Combined effect of cytokinins on multiple shoot production from cotyledonary node explants of Bauhinia vahlii. Plant Cell Tissue Organ Cult. 2000. Vol. 62, no. 1, pp. 79–83. Retrieved from:

24. Grossway A., Houck C.M., Facciotti D. Potential of micromanipulation techniques for plant improvement. Nuclear techniques and in vitro culture for plant improvement: Proc. Int. Symp. on Nuclear Techniques and In Vitro Culture for Plant Improvement (Vienna, 19–23 Aug. 1985). Vienna, IAEA, 1986, pр. 471-475.

25. Badr, A., Desjardins, Y. Sugar uptake and metabolism in tissue cultured potato plantlets cultured in liquid medium. Acta Hortic. 2007, vol. 748, pp. 265–273. Retrieved from:

26. de Klerk, G.J. Stress in plants cultured in vitro. Prop. Ornam. Plants. 2007, Vol. 7, no. 3, pp. 129–137.

27. Riabovol, L.O. (2003). Klonalne mikrorozmnozhennia roslyn: metodychni rekomendatsii dlia provedennia laboratorno-praktychnykh zaniat z «Biotekhnolohii roslyn» [Clonal micropropagation of plants: methological recommendations for laboratory and practical classes on ‘Plant Biotechnology’]. Uman, UDAA, 18 p.

28. Kushnir, H.P., Sarnatska, V.V. (2005). Mikroklonalne rozmnozhennia roslyn. Teoriia i praktyka: monohrafiia [Clonal micropropagation of plants]. Kyiv, Naukova dumka, pp. 242–269.

29. Roik, M.V., Kurylo, V.L., Voitovska, V.I. et al. (2013). Klonalne mikrorozmnozhennia miskantusu: metodychni rekomendatsii [Clonal micropropagation of miscanthus: methological recommendations]. Kyiv, Nilan-LTD, 24 p.

30. Voitovska, V.I., Storozhyk, L.I., Nediak, T.M., Prysiazhniuk, O.I., Kovalchuk, N.S. (2016). Vyznachennia stiikosti roslyn do dii alelopatychno aktyvnykh rechovyn sorho tsukrovoho: metodychni rekomendatsii [Determination of plant resistance to the action of alylopathic active substances of sugar sorghum]. Kyiv, Nilan-LTD, 20 p.

31. Voitovska, V.I., Necheporenko, L.P., Nediak, T.M. (2013). Sposib klonalnoho mikrorozmnozhennia vivsa [A method of clonal micropropagation of oat]. Patent of Ukraine, no. 85558.

32. Hazarika, B.N. Morpho-physiological disorders in in vitro cultured plants. Sci. Hortic. 2006, Vol. 108, no. 2, pp. 105–120. Retrieved from:

33. Hlybovets, A.O., Lashuk, S.O., Mazurets, L.M. (2012). Vplyv sterylizuiuchykh ahentiv, rehuliatoriv rostu, vuhlevodiv i heliuiuchykh ahentiv na reheneratsiiu povnotsinnykh roslyn khmeliu pry mikroklonalnomu rozmnozhenni [Influence of sterilizing agents, growth regulators, carbohydrates and gelling agents on regeneration of full hops plants during microclonal propagation]. Plant Varieties Studying and Protection, no. 3, pp. 74–76. Retrieved from:

34. Voitovska, V.I., Storozhyk, L.I., Nediak, T.N. (2013). Optimizatsiya usloviy deponirovaniya sorgo sakharnogo v kul'ture in vitro [Optimization of conditions for the deposition of sugar sorghum in vitro]. Nauchnoe obespechenie kartofelevodstva, ovoshchevodstva i bakhchevodstva: dostizheniya i perspektivy: sb. nauch. tr. Mezhdunar. nauch.-prakt. konf. [Scientific provision of potato, vegetable and melon growing: achievements and prospects]. Almaty, Kazakhstan, pp. 170–173.

35. Bieliaieva, O.H., Yurchenko, S.O. (2010). Kultyvuvannia roslynnoho materialu in vitro dlia pryskorennia rozmnozhennia roslyn. [Cultivation of plant material in vitro to accelerate plant reproduction] Naukovi pratsi Poltavskoi DAA [Scientific papers of Poltava State Agricultural Academy]. Enerhozberezhennia ta alternatyvni dzherela enerhii: problemy i shliakhy yikh vyrishennia [Energy Saving and Alternative Energy Sources: Problems and Solutions], Vol. 7, pp. 273–276.

36. Riabovol, L.O. (2009). Rozrobka biotekhnolohichnykh metodiv i vykorystannia yikh dlia stvorennia vykhidnoho selektsiinoho materialu tsykoriiu koreneplidnoho (Cichorium intybus L.) ta buriakiv tsukrovykh (Beta vulgaris L.). Avtoref. dys. d-ra s.-h. nauk [Development of biotechnological methods and their application in obtaining initial breeding materials of chicory root (Cichorium intybus L.) and sugar beet (Beta vulgaris L.)]. Kyiv, 40 p.


Download this article: 
PDF icon storogik-agro-1-2018-50-59.pdf658.33 KB