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Adaptation of Hydrangea L. regenerant plants to ex vitro conditions

One of the main and most challenging stages of microclonal propagation is the transfer of plants from in vitro to ex vitro conditions. Microshoots formed in in vitro culture must adapt to new environmental conditions that differ significantly in air humidity, light intensity, temperature fluctuations, and the risk of pathogen infection. Therefore, when transferring regenerated plants to non-sterile conditions, it is necessary to provide optimal growing conditions and ensure their gradual adaptation to the new environment. Regenerated plants of the genus Hydrangea intended for further cultivation were transplanted from test tubes into peat pellets. The plants were cultivated in special chambers under controlled conditions, after which they were transplanted into containers and transferred to greenhouse benches for further growth and adaptation. The study was conducted using three cultivars/ species of the genus Hydrangea: H. macrophylla ‘Nikko Blue’, H. arborescens ‘Annabelle’, and H. paniculata ‘Grandiflora’. It was determined that substrate composition had a significant effect on the survival of regenerated plants. During further cultivation, the plants were transplanted into containers filled with multicomponent substrate mixtures. The highest survival rate (88.3 %) was obtained when plants were grown in a substrate consisting of 50 % forest soil, 20 % high-moor peat, 20 % river sand, and 10 % perlite. The lowest survival rate was observed in plants grown in forest soil alone (control), amounting to only 16.9 %. When 40 % river sand and 10 % perlite were added to the forest soil, the survival rate increased by 35.8 %, while the addition of 30 % high-moor peat and 10 % river sand increased the survival rate by 54.8 % compared with the control. The increased plant survival may be associated with improved substrate aeration and drainage, which provided optimal conditions for further plant development.

Key words: explant, regenerant plant, substrate, acclimatisation, survival rate.

М. Osipov https://orcid.org/0000-0001-7004-1164


Polishchuk V. https://orcid.org/0000-0001-8157-7028


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2026
AGROBIOLOGY №1 2026