You are here

Formation of the content and quality of grain gluten in spelt-like chernobyl radiomutants of Triticum L.

In the conditions of scientific 10-year crop rotation No. 1 of the Bila Tserkva Research and Breeding Station of the Institute of Bioenergy Crops and Sugar Beet of the NAAS, 10 spelt-like Chernobyl radiomutants – RM-1–10 of winter wheat were studied. The tested samples RM-1–10 were obtained as a result of chronic irradiation in sowing in 1986 and self-sowing in 1987 in agrophytocenoses of agricultural lands within 20 kilometers of the Chernobyl NPP exclusion zone. The declared area of the experimental plot of one sample is10 m2 , replication is triple. The predecessor was peas. The seeds were sown at the optimal time for the crop with a «SD-10C» seeder. For comparison, the standard of the Forest-Steppe of Ukraine – the variety «Lisova Pisnya» was used. The aim of the research was to determine the gluten content and its quality indicators in spelt-like Chernobyl radiomutants winter wheat samples. Gluten content was determined by infrared spectroscopy (FOOS Infratec 1241 Grain Analyzer device). Gluten quality was determined according to the methodology for conducting a qualification examination of plant varieties for suitability for distribution in Ukraine according to the indicators of the GDD-1 device. The extensibility and elasticity of raw gluten were also determined. The highest gluten content on average over the years of research was noted in RM-2 and RM-9 (32.0 %), which formed an indicator at the level of the Forest Song standard – 32.4 %. The greatest variability of gluten content was established in RM-3, RM-1, RM-4, RM-2 with an indicator of the coefficient of variation of 22.2, 20.6, 20.0 and 16.5 % respectively. The lowest variability was observed in RM-7 with a difference between the highest and lowest gluten content in 2016 – 2019 of 3.8 % and a negligible coefficient of variation of 7.1 %. In terms of the gluten deformation index, on average for 2016–2019, the first (good) and second (satisfactorily strong) gluten quality groups were not noted among the studied samples. A satisfactorily weak second quality group was formed by all the studied samples and the «Lisova Pisnya» standard. According to the ability of raw gluten to stretch in length, on average for 2016–2019, all the studied RM samples (16.5–19.5 cm) exceeded the «Lisova Pisnya» standard (16.3 cm). The elasticity of raw gluten of the «Lisova Pisnya» standard (4 points) exceeded the radio mutant RM-6 (4.5 points) and RM-2 (4.3 points). At the standard level, gluten elasticity was noted in RM-1, RM-5, RM-7, RM-8.

Key words: winter wheat, spelt-like radiomutants, gluten content, gluten quality, gluten extensibility, gluten elasticity, homeostasis, breeding value.

Reference: 
1. Kolomiiets, L.A., Khomenko, S.O., Kyrylenko, V.V., Derhachov, O.L., Basanets, H.S. (2009). Statystychni parametry adaptyvnosti za urozhainistiu novykh henotypiv pshenytsi miakoi ozymoi [Statistical parameters of adaptability by yield of new genotypes of soft winter wheat]. Visnyk Ukrainskoho tovarystva henetykiv i selektsioneriv [Bulletin of the Ukrainian Society of Geneticists and Breeders]. Vol. 7, no. 2, pp. 198–205.
2. Burdeniuk-Tarasevych, L.A., Lozinskyi, M.V., Dubova, O.A. (2015). Osoblyvosti formuvannia dovzhyny stebla u selektsiinykh nomeriv pshenytsi ozymoi v zalezhnosti vid yikh henotypiv ta umov vyroshchuvannia [Peculiarities of stem length formation in winter wheat breeding numbers depending on their genotypes and growing conditions]. Ahrobiolohiia [Agrobiology]. no. 1(117), pp. 11–15.
3. Marukhniak, A.Ya., Terletska, M.I., Prudiak, L.S. (2019). Klasternyi rozpodil henotypiv vivsa za ekolohichnoiu adaptyvnistiu kilkisnykh oznak produktyvnosti [Cluster distribution of oat genotypes by ecological adaptability of quantitative traits of productivity]. Peredhirne ta hirske zemlerobstvo i tvarynnytstvo [Foothill and mountain agriculture and animal husbandry]. no. 65, pp. 77–90.
4. Kyrylenko, V.V. (2014). Tradytsiini ta suchasni metody selektsii Triticum aestivum L. u Myronivskomu instytuti pshenytsi imeni V.M. Remesla [Traditional and modern methods of breeding Triticum aestivum L. at the V.M. Remesla Myroniv Wheat Institute]. Sortovyvchennia ta okhorona prav na sorty roslyn [Variety study and protection of rights to plant varieties]. no. 4(25), pp. 41–46.
5. Shuvar, A.M., Behen, L.L., Tymkiv, M.Yu., Voitovych, R.M. (2018). Formuvannia vrozhaiu i yakosti zerna pshenytsi ozymoi zalezhno vid strokiv sivby ta rivnia zhyvlennia [Formation of yield and quality of winter wheat grain depending on sowing dates and nutrition level]. Peredhirne ta hirske zemlerobstvo i tvarynnytstvo [Foothill and mountain agriculture and livestock]. no. 63, pp. 161–173.
6. Zhemela, H.P., Barabolia, O.V., Tatarko, Yu.V., Antonovskyi, O.V. (2020). Vplyv sortovykh osoblyvostei na yakist zerna pshenytsi ozymoi [Influence of varietal characteristics on the quality of winter wheat grain]. Scientific Progress & Innovations. no. 3, pp. 32–39.
7. Zhemela, H.P., Musatov, A.H. (1989). Ahrotekhnichni osnovy pidvyshchennia yakosti zerna [Agrotechnical foundations of improving grain quality]. Kyiv, Harvest, 160 p.
8. Kozechko, V.I. (2014). Vplyv tekhnolohichnykh pryiomiv vyroshchuvannia na formuvannia pokaznykiv yakosti zerna pshenytsi ozymoi v umovakh pivnichnoho Stepu [The influence of technological methods of cultivation on the formation of quality indicators of winter wheat grain in the conditions of the northern Steppe]. Scientific Progress & Innovations. no. 2, pp. 67–73.
9. Nadew, B.B. (2018). Effects of climatic and agronomic factors on yield and quality of bread wheat (Triticum aestivum L.) seed: a review on selected factors. Advances in Crop Science and Technology. Vol. 6, Issue 2, 356 p.
10. Lozinskyi, M.V. (2006). Dobir u hibrydnykh pokolinniakh pshenytsi ozymoi za vmistom i yakistiu kleikovyny [Selection in hybrid generations of winter wheat for gluten content and quality]. Visnyk Bilotserkivskoho derzhavnoho ahrarnoho universytetu [Bulletin of the Bila Tserkva State Agrarian University]. no. 35, pp. 78–85.
11. Moskalets, T.Z., Moskalets, B.V., Didenko, C.Yu. (2015). Rezultaty selektsii pshenytsi miakoi ozymoi na polipshennia ekoloho-adaptyvnykh vlastyvostei i yakisnykh parametriv zerna [Results of breeding soft winter wheat to improve ecological and adaptive properties and grain quality parameters]. Visnyk Umanskoho natsionalnoho universytetu sadivnytstva [Bulletin of the Uman National University of Horticulture]. no. 1, pp. 58–63.
12. Demydov, O.A., Kyrylenko, V.V., Humeniuk, O.V. (2022). Metod hibrydyzatsii u selektsii Triticum aestivum L. v umovakh Tsentralnoho Lisostepu Ukrainy: monohrafiia. [Hybridization method in breeding Triticum aestivum L. in the conditions of the Central Forest-Steppe]. Kyiv, Komprynt, 267 p.
13. Karpenko, V.P., Mostoviak, I.I., Liubych, V.V., Polianetska, I.O., Petrenko, V.V. (2015). Tekhnolohichna otsinka kleikovyny zerna speltopodibnykh form pshenytsi [Technological assessment of gluten of spelt-like wheat grains]. Zbirnyk naukovykh prats Natsionalnoho naukovoho tsentru Instytut zemlerobstva NAAN [Collection of scientific papers of the National Scientific Center Institute of Agriculture of the National Academy of Sciences]. no. 2, pp. 192–198.
14. Kyrylchuk, A.M., Kovalchuk, S.O. (2021). Selektsiia na kilkisni ta yakisni pokaznyky pshenytsi ozymoi (Triticum aestivum L.). Rozshyrennia henetychnoho riznomanittia kulturnoi pshenytsi [Selection for quantitative and qualitative indicators of winter wheat (Triticum aestivum L.). Expansion of genetic diversity of cultivated wheat]. Ahroekolohichnyi zhurnal [Agroecological Journal]. no. 2, pp. 140–148.
15. Hospodarenko, H.M. (2016). Pshenytsia spelta [Spelt wheat]. Kyiv, TShchV «Sik Hrup Ukraina», 312 p.
16. Hlevaskyi, V.I., Lozinskyi, M.V., Sydorova, I.M, Shokh, S.S., Dubovyk, N.S., Kuianov, V.V. (2021). Tekhnolohiia zberihannia ta pererobky produktsii roslynnytstva: praktykum dlia zdobuvachiv pershoho (bakalavrskoho) rivnia vyshchoi osvity zi spetsialnosti 201–Ahronomiia [Technology of storage and processing of crop products: a workshop for applicants of the first (bachelor's) level of higher education in the specialty 201–Agronomy]. Bila Tserkva, 175 p.
17. Hasanova, I.I., Kryvoruchko, N.L. (2012). Yakist zerna novykh sortiv pshenytsi ozymoi v pivnichnomu Stepu Ukrainy: materialy VIII Mizhnarodnoi naukovo-praktychnoi konferentsii «Nainovyte nauchny postyzhenyia – 2012» [Grain quality of new varieties of winter wheat in the northern Steppe of Ukraine: Proceedings of the VIII International Scientific and Practical Conference “Latest Scientific Achievements – 2012”]. Bolharia, pp. 40–42.
18. Konoplova, Ye.L. (2012). Efektyvnist zakhodiv pidvyshchennia urozhainosti ta yakosti zerna pshenytsi ozymoi po poperednyku chornyi par u pivnichnomu Stepu Ukrainy [Effectiveness of measures to increase the yield and quality of winter wheat grain using the precursor black par in the northern Steppe of Ukraine]. Biuleten Instytutu silskohohospodarstva stepovoi zony NAAN Ukrainy [Bulletin of the Institute of Agriculture of the Steppe Zone of the NAAS of Ukraine]. Dnipropetrovsk, New ideology, no. 3, pp. 99–103.
19. Konoplova, Ye.L. (2012). Efektyvnist vyroshchuvannia pshenytsi ozymoi zalezhno vid tekhnolohichnykh zakhodiv v pivnichnomu Stepu Ukrainy [Effectiveness of winter wheat cultivation depending on technological measures in the northern Steppe of Ukraine]. Ahrobiolohiia [Agrobiology]. no. 7(91), pp. 117–120.
20. Solodushko, M.M., Hasanova, I.I., Sereda, I.I. (2012). Vplyv mineralnoho zhyvlennia na yakist zerna pshenytsi ozymoi v pivnichnomu Stepu: materialy naukovo-praktychnoi konferentsii molodykh uchenykh i spetsialistiv: «Ahrotekhnolohii dlia staloho vyrobnytstv konkurentospromozhnoi produktsii» [The influence of mineral nutrition on the quality of winter wheat grain in the northern Steppe: materials of the scientific and practical conference of young scientists and specialists: "Agrotechnologies for sustainable production of competitive products"]. Chabany, pp. 61–62.
21. Vasyliuk, P.M. (2014). Otsinka stabilnosti ta plastychnosti pokaznykiv produktyvnosti ta yakosti novykh sortiv pshenytsi miakoi ozymoi v umovakh Lisostepu Ukrainy [Assessment of stability and plasticity of productivity and quality indicators of new varieties of soft winter wheat in the conditions of the Forest-Steppe of Ukraine]. Sortovyvchennia ta okhorona prav na sorty roslyn [Varietal study and protection of rights to plant varieties]. no. 1(22), pp. 15–18.
22. Poperelia, F.O. (1996). Try osnovni henetychni systemy yakosti zerna ozymoi miakoi pshenytsi [Three main genetic systems of grain quality of winter soft wheat]. Realizatsiia potentsiinykh mozhlyvostei sortiv ta hibrydiv Selektsiino-henetychnoho instytutu v umovakh Ukrainy: zb. nauk. prats [Realization of potential possibilities of varieties and hybrids of the Breeding and Genetic Institute in the conditions of Ukraine: collection of scientific works]. Odesa, pp. 117–132.
23. Zhyvotkov, L.O., Korchynskyi, A.A. (2000). Formuvannia sortovoi struktury pshenytsi [Formation of wheat varietal structure]. Visnyk ahrarnoi nauky [Bulletin of Agrarian Science]. no. 7, pp. 42–43.
24. Metodyka provedennia kvalifikatsiinoi ekspertyzy sortiv roslyn na prydatnist do poshyrennia v Ukraini. Metody vyznachennia pokaznykiv yakosti produktsii roslynnytstva [Methodology for conducting qualification examination of plant varieties for suitability for distribution in Ukraine. Methods for determining quality indicators of crop production]. Kyiv, 2016, 158 p.
25. Zhyvotkov, L.O., Biriukov, S.V. (1993). Ozymi zernovi kultury [Winter grain crops]. Kyiv, Harvest, 288 p.
26. Hladchenko, V.Ya., Fabrychnikova, I.A. (2012). Vyznachennia kilkosti i yakosti zerna pshenytsi: metodychni vkazivky [Determination of the quantity and quality of wheat grain: methodological guidelines]. Kharkiv, KhNTUSH im. P. Vasylenka, 16 p.
27. Zhang, X., Zhang, B., Wu, H. (2018). Effect of high-molecular-weight glutenin subunit deletion on soft wheat quality properties and sugar-snap cookie quality estimated through near-isogenic lines. Journal of Integrative Agriculture. Vol. 17, Issue 5, pp. 1066–1073.
28. Zhemela, H.P., Barabolia O.V. (2011). Tekhnolohiia boroshnomelnoho ta krupianoho vyrobnytstva [Technology of flour milling and cereal production]. Poltava, 292 p. 29. Soroka, V.I., Ulych, L.I., Vasyliuk, P.M., Khakhula, V.S. (2011). Efektyvne vykorystannia selektsiino-henetychnoho potentsialu sortiv pshenytsi ozymoi miakoi [Effective use of breeding and genetic potential of soft winter wheat varieties]. Ahrobiolohiia [Agrobiology]. no. 6, pp. 13–19.
30. Zhemela, H.P., Kuznietsova, O.A. (2012). Vplyv sortovykh vlastyvostei na produktyvnist ta yakist zerna pshenytsi [Influence of varietal properties on the productivity and quality of wheat grain]. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii [Bulletin of the Poltava State Agrarian Academy]. no. 3, pp. 23–25.
31. Amagai, Y., Burdenyuk-Tarasevych, L.A., Goncharov, N.P., Watanabe, N. (2017). Microsatellite mapping of the loci for false glume and semi-compact spike in Triticum L. Genetic Resources and Crop Evolution. no. 64(8), pp. 2105–2113.
32. Tkachyk, S.O., Kyienko, Z.B., Prysiazhniuk, L.M. (2016). Metodyka provedennia kvalifikatsiinoi ekspertyzy sortiv roslyn na prydatnist do poshyrennia v Ukraini. Metody vyznachennia pokaznykiv yakosti produktsii roslynnytstva [Methodology for conducting qualification examination of plant varieties for suitability for distribution in Ukraine. Methods for determining quality indicators of plant products]. Vinnytsia, 159 р.
33. Oliinyk, K.M., Blazhevych, L.Yu., Davydiuk, H.V. (2018). Vplyv adaptyvnykh tekhnolohii vyroshchuvannia na pokaznyky yakosti zerna pshenytsi ozymoi [The influence of adaptive cultivation technologies on the quality indicators of winter wheat grain]. Kormy i kormovyrobnytstvo [Feed and feed production]. no. 86, pp. 141–146.
34. Nevmyvako, H.V. (2008). Vplyv poperednykiv na vrozhainist i yakist zerna ozymoi pshenytsi [The influence of predecessors on the yield and quality of winter wheat grain]. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii [Bulletin of the Poltava State Agrarian Academy]. no. 4, pp. 74–76.
35. DSTU 3768:2019. Pshenytsia. Tekhnichni umovy [DSTU 3768:2019. Wheat. Technical conditions]. Available at: https://zakon.isu.net.ua/sites/default/files/normdocs/dstu_3768_2019.pdf
36. Tkachyk, S.O. (2015). Tekhnolohichna otsinka roslynnytskoi produktsii sortiv silskohospodarskykh vydiv. Metodyka derzhavnoi naukovo-tekhnichnoi ekspertyzy sortiv roslyn. Metody vyznachennia pokaznykiv yakosti produktsii roslynnytstva [Technological assessment of crop production of agricultural varieties. Methodology of state scientific and technical examination of plant varieties. Methods for determining the quality indicators of crop production]. Vinnytsia, LLC «Nilan-LTD», рр. 6–64.
37. Rybalka, O.I. (2011). Yakist pshenytsi ta yii polipshennia: monohrafiia [Wheat quality and its improvement]. Kyiv, Lohos, 495 p.
Download this article: 
AttachmentSize
PDF icon dolgaliova_1_2025.pdf1.39 MB