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Effect of pre-sowing treatment of soybean seeds with biopreparations on the photosynthetic pigments content and yield under unstable moisture conditions and organic farming

Global soybean production is continuously increasing, as it remains one of the most in-demand crops on the global food market. At the same time, climate changes, manifested through rising temperatures and precipitation deficits during the crop's vegetative period, significantly affects its yield. This necessitates the implementation of new technological solutions that ensure the efficient use of production resources and the safety of the final product. One of such promising methods is the pre-sowing treatment of soybean seeds with biopreparations, which enhance plant resistance to stress factors and contribute to achieving potential yields with high quality. The aim of this study is to determine the effect of presowing inoculation and treatment with a phytohormonal preparation on the content of photosynthetic pigments and yield of soybeans in organic farming system in challenging climate conditions. Field experiments conducted in 2022–2024 in Poltava region investigated the effect of pre-sowing seed treatment of the «Horol» soybean variety with the inoculant Legume Fix and the phytohormonal preparation «Violar» on leaf area, photosynthetic pigment content, and yield under various weather conditions within an organic farming system. The results showed that pre-sowing seed treatment contributed to an increase in leaf area by an average of 13.4 % with «Legume Fix» and 19.3 % with «Violar». It was established that treatment with «Violar» led to an increase in the concentration of Chl a, Chl b, and total Chl (a+b) compared to the control by an average of 18.9 %, 13.3 %, and 17.2 % respectively, whereas inoculation with «Legume Fix» increased these indicators by 7.4 %, 3.4 %, and 6.2 % respectively. It was also determined that pre-sowing seed treatment with «Legume Fix» and «Violar» resulted in an average yield increase of 13.2 % and 20.6 % respectively, compared to the control plants. A strong direct correlation was calculated between leaf area, Chl (a+b) content, and yield levels across all study variants: control (0.990; 0.986), «Legume Fix» (0.996; 0.983), and «Violar» (0.986; 0.988) respectively.

Key words: Glycine max L. Merr., organic farming, inoculation, leaf area, photosynthetic pigments, weather conditions.

 

Reference: 
1. Bosanquet, J. Summary series: soy. Available at: https://tabledebates.org/building-blocks/table-summary-series-soy
2. Thrane, M., Paulsen, P.V., Orcutt, M.W., Krieger, T.M. (2017). Soy protein: Impacts, production, and applications. Sustainable protein sources. Academic Press. pp. 23–45. Available at: http://www.sciencedirect.com/science/article/pii/ B9780128027783000020.
3. Soybean Market Size & Share Analysis – Growth Trends & Forecasts (2024–2029). Available at: https://www.mordorintelligence.com/industry-reports/soybean-market
4. IGS: Global soybean production in 2024/25 MY to break record. Available at: https://www.tridge.com/news/igs-global-soybean-production-in202425-my-t-...
5. Ukraine soybean area, yield and production. Available at: https://ipad.fas.usda.gov/countrysummary/Default.aspx?id=UP&crop=Soybean
6. Polevyk, V. (2024). Ukraina stala draiverom z vyroshchuvannia soi [Ukraine has become a driver in soybean cultivation]. Available at: https://agroportal.ua/news/rastenievodstvo/ukrajina-stala-drayverom-z-vi...
7. Voora, V., Larrea, C., Huppe, G., Nugnes, F. (2022). IISD’s State of Sustainability Initiatives Review: Standards and investments in sustainable agriculture. Canada, International Institute for Sustainable Development. Available at: https://www. researchgate.net/publication/378140518_State_of_ Sustainability_Initiatives-Review-Standards_and_ Investments_in_Sustainable_Agriculture
8. Rotundo, J.L., Marshall, R., McCormick, R., Truong, S.K., Styles, D., Gerde, J.A., Gonzalez-Escobar, E., Carmo-Silva, E., Janes-Bassett, V., Logue, J., Annicchiarico, P., de Visser, C., Dind, A., Dodd, I.C., Dye, L., Long, S.P., Lopes, M.S., Pannecoucque, J., Reckling, M., Rushton, J., Schmid, N., Shield, I., Signor, M., Messina, C.D., Rufino, M.C. (2024). European soybean to benefit people and the environment. Scientific Reports. no. 14, 7612. DOI: 10.1038/ s41598-024-57522-z
9. Nandi, P. (2017). Organic soybean market research report by application (crush, food use, feed use) and by region (North America, Europe, AsiaPacific, and rest of the world) – market forecast till 2030. 89 p. Available at: https://www.marketresearchfuture.com/reports/organic-soybean-market-4208.
10. Chayka, T.O., Ponomarenko, S.V. (2015). Tekhnoloho-ekonomichni osoblyvosti vyroshchuvannia orhanichnoi soi ta ozymoi pshenytsi na furazh [Technological and economic features of growing organic soybeans and winter wheat forage]. Visnyk Umanskoho natsionalnoho universytetu sadivnytstva [Bulletin of Uman national university of horticulture]. no. 1, pp. 100–106.
11. Chaika, T.O. (2023). Vyroshchuvannia orhanichnoi soi v Ukraini: perspektyvy ta realnist [Organic soybean cultivation in Ukraine: prospects and reality]. Aktualni napriamky ta problematyka u tekhnolohiiakh vyroshchuvannia produktsii roslynnytstva: mizhnar. nauk.-prakt. konf. [Current trends and challenges in crop production technologies: international scientific and practical conference]. Poltava, PDAU, pp. 10–12.
12. Hossain, M.S., Khan, M.A.R., Mahmud, A., Ghosh, U.K., Anik, T.R., Mayer, D., Das, A.K., Mostofa, M.G. (2024). Differential drought responses of soybean genotypes in relation to photosynthesis and growth-yield attributes. Plants. no. 13(19), 2765. DOI: 10.3390/ plants13192765
13. Nakagawa, A.C.S., Ario, N., Tomita, Y., Tanaka, S., Murayama, N., Mizuta, C., Iwaya-Inoue, M., Ishibashi, Y. (2020). High temperature during soybean seed development differentially alters lipid and protein metabolism. Plant Production Science. no. 23(4), pp. 504–512. DOI: 10.1080/1343943X.2020.1742581
14. Siebers, M.H., Yendrek, C.R., Drag, D., Locke, A.M., Acosta, L.R., Leakey, A.D.B., Ainsworth, E.A., Bernacchi, C.J., Ort, D.R. (2015). Heat waves imposed during early pod development in soybean (Glycine max) cause significant yield loss despite a rapid recovery from oxidative stress. Global Change Biology. no. 21(8), pp. 3114–3125. DOI: 10.1111/gcb.12935
15. Lobell, D.B., Asner, G.P. (2003). Climate and management contributions to recent trends in U.S. agricultural yields. Science. no. 299(5609), 1032. DOI: 10.1126/science.1077838
16. Puteh, A., ThuZar, M., Mondal, M.M.A, Abdullah, N.A.P.B., Halim, M.R.A. (2013). Soybean [Glycine max (L.) Merrill] seed yield response to high temperature stress during reproductive growth stages. Australian Journal of Crop Science. no. 7(10), pp. 1472–1479. DOI: 10.3316/informit.618691
17. Jumrani, K., Bhatia, V.S. (2018). Impact of combined stress of high temperature and water deficit on growth and seed yield of soybean. Physiology and Molecular Biology of Plants. no. 24(1), pp. 37–50. DOI: 10.1007/s12298-017-0480-5
18. Ogunkanmi, L., MacCarthy, D.S., Adiku, S.G.K. (2022). Impact of extreme temperature and soil water stress on the growth and yield of soybean (Glycine max (L.) Merrill). Agriculture. no. 12(1), 43. DOI: 10.3390/agriculture12010043
19. Khan, M.N., Zhang, J., Luo, T., Liu, J., Rizwan, M., Fahad, S., Xu, J., Hu, L. (2019). Seed priming with melatonin coping drought stress in rapeseed by regulating reactive oxygen species detoxification: Antioxidant defense system, osmotic adjustment, stomatal traits and chloroplast ultrastructure perseveration. Industrial Crops and Products. no. 140, 111597. DOI: 10.1016/j.indcrop.2019.111597
20. Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G.A., Sajid, M., Subtain, M.U., Shabbir, I. (2013). Seed priming a technique. International Journal of Agriculture and Crop Sciences. no. 6(20), pp. 1373–1381.
21. Korotkova, I.V., Chaika, Т.O., Romashko, Т.P., Chetveryk, О.O., Rybalchenko, А.M., Barabolia, O.V. (2023). Emmer wheat productivity formation as depending on pre-sowing seed treatment method in organic and traditional technology cultivation. Regulatory Mechanisms in Biosystems. no. 14(1), pp. 41–47. DOI: 10.15421/022307
22. Marinkovic, J., Bjelic, D., Tintor, B., Miladinovic, J., Dukic, V., Dordevic, V. (2018). Effects of soybean co-inoculation with plant growth promoting rhizobacteria in field trial. Romanian Biotechnological Letters. no. 23(2), pp. 13401–13408.
23. Legumefix. 100% Organic Farm-Applied Peat-Based Inoculant. Available at: https://legumetechnology.co.uk/products/legumefix/
24. Tsygankova, V., Shysha, E., Galkin, A., Biliavska, L., Iutynska, G., Yemets, A., Blume, Y. (2017). Impact of microbial biostimulants on induction of callusogenesis and organogenesis in the isolated tissue culture of wheat in vitro. Journal of the Mechanics and Physics of Solids. no. 5(3), pp. 155–164.
25. Easlon, H.M., Bloom, A.J. (2014). Easy Leaf Area: Automated digital image analysis for rapid and accurate measurement of leaf area. Applications in Plant Sciences. no. 2(7), 1400033. DOI: 10.3732/apps.1400033
26. Wellburn, A.R. (1994). The spectral determination of chlorophylls a, and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology. no. 144(3), pp. 307–313.
27. Hadzovskyi, H.L., Novytska, N.V., Martynov, O.M. (2019). Vmist khlorofilu v lysti roslyn i vrozhainist soi pry vnesenni khelatnykh mikroelementiv [Chlorophyll content in the leaves of plants and the yield of soybeans with the introduction of chelated micronutrients]. Tavriiskyi naukovyi visnyk. Silskohospodarski nauky [Taurida Scientific Herald. Rural Sciences]. no. 105, pp. 34–38.
28. Chaika, T.O., Liashenko, V.V., Khomenko, B.S. (2023). Vplyv inokuliatsii nasinnia na vrozhainist soi za orhanichnoi tekhnolohii vyroshchuvannia [The impact of seed inoculation on soybean yield under organic cultivation technology]. Tavriiskyi naukovyi visnyk. Silskohospodarski nauky [Taurida Scientific Herald. Rural Sciences]. no. 133, pp. 180–187. DOI: 10.32782/2226-0099.2023.133.24
29. Nimenko, S.S., Grabovskyi, M.B. (2023). Grain yield of soybean varieties depends on elements of organic growing technology. Irrigated farming. no. 79, pp. 52–59. DOI: 10.32848/0135-2369.2023.79.7
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