You are here

Головна » 2017 » AGROBIOLOGY №1 2017

THE ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OBTAINED FROM LEAVES OF COELOGYNE BRACHYPTERA RCHB. F. (ORCHIDACEAE)

A number of medicinal orchids have been screened for antimicrobial activity in recent years. As a result, many scientists have reported antimicrobial properties of various orchid species, including Coelogyne spp. The aim of the present study was to investigate the antibacterial effects of ethanolic extract obtained from Coelogyne brachyptera Rchb.f. leaves against specific Gram-positive (Staphylococcus aureus ATCC 25923 and methicillin-resistant S. aureus locally isolated) and Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853, metallo-beta-lactamases (MβL)-positive Pseudomonas aeruginosa locally isolated, Escherichia coli ATCC 25922, Salmonella enteriditis locally isolated). Our results showed that the ethanolic extract of C. brachyptera leaves showed strong activity against the Gram-positive bacterial strains (20 mm diameter of inhibition zone for S. aureus and 26.5 mm for meticillin-resistant S. aureus), and moderate activity against Gram-negative bacteria (18.2 mm for E. coli, 16.5 mm for P. aeruginosa and 18.3 mm for (MβL) P. aeruginosa, and 14.8 mm for S. enteriditis). The present study revealed the good antimicrobial activities of ethanolic extract obtained from leaves of C. brachyptera. A Gram-positive strains (S. aureus and meticillin-resistant S. aureus) were more susceptible to the ethanolic leaf extracts of C. brachyptera as compared to Gram-negative bacteria. Beside, extract of C. brachyptera leaves has displayed strong inhibitory effect against S. aureus and meticillin-resistant S. aureus. To conclude, further work is required to find out the active substances from C. brachyptera plant extracts and to carry out pharmacological studies.

Key words: Coelogyne brachyptera, leaves, extract, antimicrobial activity, paper disc diffusion method.

 

BUYUN L.


TKACHENKO H.


OSADOWSKI Z.


KOVALSKA L.


GYRENKO O.


Reference: 
  1. Averyanov L.V., Phan Ke Lock, Nguyen Tien Hiep, Harder D.K. Phytogeographic review of Vietnam and adjacent areas of Eastern Indochina. Komarovia. Vol. 3, рр. 1-83.
  2. Bauer A.W., Kirby W.M., Sherris J.C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966, vol. 45(4), рр. 493-496.
  3. Buyun L., Tkachenko H., Kovalska L., Osadowski Z. Preliminary screening of Coelogyne ovalis Lindl. (Orchidaceae) for antimicrobial activity against Staphylococcus aureus. Dni laboratornoy meditsiny: sbornik materialov Respublikanskoy nauchno-prakticheskoy konferentsii / otv. red. V. V. Vorobev. – Grodno: GrGMU, 2016, 10 р.
  4. Buyun L., Tkachenko H., Osadowski Z. Antimicrobial activities of the various extracts obtained from leaves and pseudobulbs of Coelogyne tomentosa Lindl. (Orchidaceae). III mizhnarodna naukovo-praktychna konferentsiya studentiv, aspirantiv i molodykh vchenykh «Ekolohiya – filosofiya isnuvannya lyudstva», prysvyachena 30-y richnytsi Chornobylskoi katastrofy, Natsionalnyi universytet bioresursiv i pryrodokorystuvannya Ukrainy, 26-28 kvitnya, 2016, Kyiv. рр. 10-12.
  5. Buyun L., Tkachenko H., Osadowski Z., Kovalska L. Antimicrobial activities of the various extracts obtained from leaves and pseudobulbs of Coelogyne huettneriana Rchb.f. (Orchidaceae). Ontohenez – stan, problemy ta perspektyvy vyvchennya roslyn v kulturnykh ta pryrodnykh tsenozakh: Mizhnar. konf., tezy dop.: Prysvyachena 110 richchyu vid dnya narodzhennya dekana ahronomichnoho fakultetu Lipesa Veniamina Elyevycha (10-11 chervnya 2016 r), Kherson, RVTs Kolos, 2016, рр. 8-11.
  6. Buyun L., Tkachenko H., Truchan M., Kovalska L., Gyrenko O. Antimicrobial screening of ethanolic extract of Coelogyne cristata Lindl. (Orchidaceae) leaves. Proceedings of the Conference «Modern Approaches to Formation and Management of Anthropogenic and natural Biocoenosis in the Countries of Eastern Europe», Section 1. Land resources and the efficiency of their use. Kherson, 2015, рр. 19-27.
  7. Chase M.W., Cameron K.M., Freudenstein J.V., Pridgeon A.M., Salazar G., van den Berg C., Schuiteman A. An updated classification of Orchidaceae. Bot. J. Linn. Soc. 2015, vol. 177, рр. 151-174.
  8. Góralczyk A., Tkachenko H., Buyun L., Osadowski Z. The antimicrobial potential of ethanolic extract of Coelogyne cristata Lindl. (Orchidaceae) leaves. Youth and Progress of Biology: Book of Abstracts of XII International Scientific Conference for Students and PhD Students (Lviv, 19-21 April 2016), рр. 259-260.
  9. Kong J.M., Khang N.G., Sail C.L., Fatt C.T. Recent advances in traditional plant drugs and orchids. Acta Pharmacology Sinca. 2003, vol. 24(1), рр. 7-21.
  10. Mitra A., Dutta S., Nath Mandal D., Bhattacharyya D., Hazra J. Chemical Characterization and Antibacterial activity of Swarna Jibanti (Coelogyne cristata Lindl.). Int. J. Ayu. Pharm. Chem. 2015, vol. 3(2), рр. 299-315.
  11. Okoth D.A., Chenia H.Y., Koorbanally N.A. Antibacterial and antioxidant activities of flavonoids from Lannea alata (Engl.) Engl. (Anacardiaceae). Phytochem. Lett. 2013, vol. 6, рр. 476-481.
  12. Pant B. Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. Afr. J. Plant Sci. 2013, vol. 7(10), рр. 448-467.
  13. Rao A.N. Medicinal orchid wealth of Arunachal Pradesh. Indian Medicinal Plants of Conservation Concern (Newsletter of ENVIS Node, Foundation for Revitalization of Local Health Traditions, Bangalore). 2004, vol. 1(2), рр. 1-5.
  14. Sahaya S.B., Chitra D.B., Moin S., Servin W.P. Evaluation of bioactive potential of Coelogyne nervosa A. Rich. – an endemic medicinal orchid of western Ghats, India. Asian J. Pharm. Clin. Res. 2013, vol. 6(S-1), рр. 114-118.
  15. Siddhartha Singh, Amit Kumar Singh, Sunil Kumar, Mukul Kumar, Pramod Kumar Pandey, Mayanglambam Chandra Kumar Singh Medicinal properties and uses of orchids: a concise review. Elixir Appl. Botany. 2012, vol. 52, рр. 11627-11634.
  16. Szlachetko D. Genera et species Orchidalium. 1. Polish Bot. J. 2001, vol. 46, рр. 11-26.
  17. Tkachenko G.M., Trukhan M.A., Buyun L.I., Shon Kh.N., Chiong M. Antibakterialnaya effektivnost nekotorykh vidov orkhidey roda Coelogyne Lindl. v otnoshenii zolotistogo stafilokokka. Materialy XI Mezhdunarodnoy (XX Vserossiyskoy) Pirogovskoy nauchnoy meditsinskoy konferentsii studentov i molodykh uchenykh. Rossiyskiy natsionalnyi issledovatelskiy meditsinskiy universitet imeni N.I. Pirogova, Moskva, 17 marta 2016, рр. 632-633.
  18. Tkachenko H., Truchan M., Buyun L., Kovalska L., Gyrenko A. Antifungal efficacy of some orchids from Coelogyne Lindl. genus against Candida albicans. Tezy dopovidey Mizhnarodnoi naukovo-praktychnoi konferentsii vchenykh, aspirantiv i studentiv «Intehrovanyi zakhyst ta karantyn roslyn. Perspektyvy rozvytku v XXI stolitti», Natsionalnyi universytet bioresursiv i pryrodokorystuvannya Ukrainy, 19-20 lystopada 2015 r., m. Kyiv, Ukraina, рр. 178-181.
  19. Zar J.H. Biostatistical Analysis. 4th ed., Prentice-Hall Inc., Englewood Cliffs, New Jersey, 1999.

 

Download this article: 
AttachmentSize
PDF icon buyn_1_2017.pdf653.66 KB
YEAR: 
2017
AGROBIOLOGY №1 2017