In vitro potato plants of Podolyanka variety were grown in modified Murashige and Skoog medium. In postaseptic conditions only mineral part of the environment was added. Soil (chornosem), peat, sphagnum moss, sand, granite dust, pearlite, crystal soil and crystal water hydrogels, plastahar were used as substrates. There has been defined the influence of  substrate and greengouse cassete cages size on the efficiency of postaseptic in vitro potato growing. The highest survival rate was noted in planting the cuttings on such substrates: sphagnum moss, pearlite, hydrogel and mineral wool. The most intense green color and large size leaf plates were in ex vitro plants, grown on mineral wool and pearlite. Cultivating the cuttings on hydrogels accelerated tuber forming. The maximum output of the regenerants among the compared substrates for in vitro sprouts planting was obtained with perlite apllying. The optimal nutrition area for minitubers obtaining was determined.

Key words: potato, substrate, regeneration, minituber, nutrition area.