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The exposure of sterilization and the selection of sterilizer for introducing of the microcuttings of the varieties of golden currant (ribes aureum pursh.) in vitro
The peculiarities of sterilization of explants are researched, sterilizer, its concentration, exposure processing of the varieties of golden currant before introducing in vitro are selected. The terms of an effective sterilization of the varieties of golden currant are selected. The optimal time for effective sterilization of various genotypes and the yield of sterile viable plant material are determined. It was found that the most effective sterilizer for explants of golden currant is a 0.1% of mercury dichloride (HgCl2), exposure of sterilization – 15 minutes. The yield of sterile material is 93%, and the yield of viable mikrocuttings - 89%. It is effective to use of sterilizer of septodor forte. The sterilization efficiency was 89%, and the viability of explants was 81% at the concentration of 2.5% and exposure 15 minutes. When the concentration of septodor forte was higher the efficiency of sterilization are increased to 95%, but the viability of explants was low - 9%.
Key words: golden currant, sterilizer, exposure, concentration, in vitro.
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